Call the Chemical Hygiene Officer when a significant spill occurs. A lab incident report form must be filled out for a significant spill (found in Appendix C). A significant spill is defined as:
- Spills greater than 5ml outside of primary containment
- Spills that result in an exposure
- Spills that present an inhalation hazard
- Spills that cannot be easily cleaned
- Spills that endanger people or the environment
SMALL SPILL (LESS THAN 1L) – DECONTAMINATION & CLEAN-UP
- Stop work and secure all items you are working with
- Replace any contaminated personal protective equipment (PPE)
- Make sure you are wearing the appropriate PPE such as disposable gloves, lab coat, and eye protection (safety goggles or glasses).
- Take absorbent pads and place over the spill area.
- Make a fresh solution of 10% bleach and pour over the absorbent pads. The absorbent pads absorb the spill, help to contain the 10% bleach and prevent splattering
- Let the bleach solution inactivate and decontaminate the biological material for 15 minutes.
- Soak up and clean up the excess bleach solution and decontaminated material with extra absorbent pads or paper towels
- Report the spill to Chemical Hygiene Officer. This is very important as Tusculum University maintain an ongoing log/list of spills and injuries as applicable and reports these as required.
- Dispose of in a biohazard container.
LARGE SPILL (GREATER THAN 1L) – DECONTAMINATION & CLEAN-UP
- Stop work immediately, secure all items and avoid inhaling airborne aerosols.
- Notify others to leave the room immediately.
- Label the area off-limits for at least 30 minutes. This allows the ventilation system to purge the air.
- Remove contaminated PPE and/or clothing, turn exposed clothing inward, and put in autoclave or biohazard container
- Wash all exposed skin with soap and water.
- Report the spill to the Chemical Hygiene Officer. This is very important as Tusculum University maintains an ongoing log/list of spills and injuries as applicable and reports these as required.
- After at least 30 minutes, Biosafety personnel will enter the area to clean up the spill.