{"id":633,"date":"2020-02-12T10:11:12","date_gmt":"2020-02-12T15:11:12","guid":{"rendered":"https:\/\/web.tusculum.edu\/safety\/?page_id=633"},"modified":"2020-02-12T10:23:04","modified_gmt":"2020-02-12T15:23:04","slug":"biological-spills-in-the-laboratory","status":"publish","type":"page","link":"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/biological-spills-in-the-laboratory\/","title":{"rendered":"BIOLOGICAL SPILLS IN THE LABORATORY"},"content":{"rendered":"<p>Call the Chemical Hygiene Officer when a significant spill occurs. A lab incident report form must be filled out for a significant spill (found in Appendix C). A significant spill is defined as:<\/p>\n<ul>\n<li>Spills greater than 5ml outside of primary containment<\/li>\n<li>Spills that result in an exposure<\/li>\n<li>Spills that present an inhalation hazard<\/li>\n<li>Spills that cannot be easily cleaned<\/li>\n<li>Spills that endanger people or the environment<\/li>\n<\/ul>\n<h3>SMALL SPILL (LESS THAN 1L) &#8211; DECONTAMINATION &amp; CLEAN-UP<\/h3>\n<ol>\n<li>Stop work and secure all items you are working with<\/li>\n<li>Replace any contaminated personal protective equipment (PPE)<\/li>\n<li>Make sure you are wearing the appropriate PPE such as disposable gloves, lab coat, and eye protection (safety goggles or glasses).<\/li>\n<li>Take absorbent pads and place over the spill area.<\/li>\n<li>Make a fresh solution of 10% bleach and pour over the absorbent pads. The absorbent pads absorb the spill, help to contain the 10% bleach and prevent splattering\n<ol>\n<li>Let the bleach solution inactivate and decontaminate the biological material for 15 minutes.<\/li>\n<li>Soak up and clean up the excess bleach solution and decontaminated material with extra absorbent pads or paper towels<\/li>\n<\/ol>\n<\/li>\n<li>Report the spill to Chemical Hygiene Officer. This is very important as Tusculum University maintain an ongoing log\/list of spills and injuries as applicable and reports these as required.<\/li>\n<li>Dispose of in a biohazard container.<\/li>\n<\/ol>\n<h3>LARGE SPILL (GREATER THAN 1L) &#8211; DECONTAMINATION &amp; CLEAN-UP<\/h3>\n<ol>\n<li>Stop work immediately, secure all items and avoid inhaling airborne aerosols.<\/li>\n<li>Notify others to leave the room immediately.<\/li>\n<li>Label the area off-limits for at least 30 minutes. This allows the ventilation system to purge the air.<\/li>\n<li>Remove contaminated PPE and\/or clothing, turn exposed clothing inward, and put in autoclave or biohazard container<\/li>\n<li>Wash all exposed skin with soap and water.<\/li>\n<li>Report the spill to the Chemical Hygiene Officer. This is very important as Tusculum University maintains an ongoing log\/list of spills and injuries as applicable and reports these as required.<\/li>\n<li>After at least 30 minutes, Biosafety personnel will enter the area to clean up the spill.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Call the Chemical Hygiene Officer when a significant spill occurs. A lab incident report form must be filled out for a significant spill (found in Appendix C). A significant spill is defined as: Spills greater than 5ml outside of primary containment Spills that result in an exposure Spills that present an inhalation hazard Spills that [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":598,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_links_to":"","_links_to_target":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>TUSCULUM COLLEGE :: BIOLOGICAL SPILLS IN THE LABORATORY<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/biological-spills-in-the-laboratory\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"TUSCULUM COLLEGE :: BIOLOGICAL SPILLS IN THE LABORATORY\" \/>\n<meta property=\"og:description\" content=\"Call the Chemical Hygiene Officer when a significant spill occurs. 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