{"id":598,"date":"2020-02-10T15:11:24","date_gmt":"2020-02-10T20:11:24","guid":{"rendered":"https:\/\/web.tusculum.edu\/safety\/?page_id=598"},"modified":"2020-03-03T10:46:56","modified_gmt":"2020-03-03T15:46:56","slug":"biosafety-manual","status":"publish","type":"page","link":"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/","title":{"rendered":"BIOSAFETY MANUAL"},"content":{"rendered":"<h2>TUSCULUM UNIVERSITY SAFETY INFORMATION AND EMERGENCY CONTACT INFORMATION<\/h2>\n<h3 style=\"display: inline !important;\">Emergency Providers<br \/>\n<div class=\"su-table su-table-alternate\"><\/h3>\n<table>\n<tbody>\n<tr>\n<td>Emergency Medical Assistance<\/td>\n<td>911<\/td>\n<\/tr>\n<tr>\n<td>Tusculum Campus Safety<\/td>\n<td>(423)636-7318<br \/>\n(423)470-2710<\/td>\n<\/tr>\n<tr>\n<td>City of Tusculum Police Department<\/td>\n<td>(423)638-6211<\/td>\n<\/tr>\n<tr>\n<td>Tusculum Volunteer Fire Department<\/td>\n<td>(423)639-3133<\/td>\n<\/tr>\n<tr>\n<td>Greeneville Fire Department<\/td>\n<td>(423)638-4243<\/td>\n<\/tr>\n<tr>\n<td>State Poison Control Center<\/td>\n<td>1-800-222-1222<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><\/div><\/h3>\n<h3>Laboratory Contact Information<\/h3>\n<div class=\"su-table su-table-alternate\">\n<table>\n<tbody>\n<tr>\n<td>Chemical Hygiene Officer<\/td>\n<td>Kim Carter<\/td>\n<td>(423)636-7300 ext. 5706<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<h3>Reporting Incidents<\/h3>\n<p>The following information will be needed when communicating with any emergency provider:<\/p>\n<ul>\n<li>Nature of the incident (description \u2013 including fire, substances involved, number of individuals involved, and their physical condition).<\/li>\n<li>Individuals involved (name, gender, age)<\/li>\n<li>Location of the incident (including who will meet any emergency vehicles and where)<\/li>\n<li>Person reporting the incident (name and phone number being used to report incident)\n<ul>\n<li>NOTE: DO NOT allow the phone to be tied up for calls unrelated to the incident<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<div class=\"su-divider su-divider-style-default\" style=\"margin:15px 0;border-width:3px;border-color:#999999\"><\/div>\n<p>In addition to this manual, the National Institutes of Health (NIH) require the lab to follow the BSL procedures as outlined in <em><a href=\"https:\/\/www.cdc.gov\/labs\/BMBL.html?CDC_AA_refVal=https%3A%2F%2Fwww.cdc.gov%2Fbiosafety%2Fpublications%2Fbmbl5%2Findex.htm\" target=\"_blank\">Biosafety in Microbiological and Biomedical Laboratories, 5th Edition<\/a><\/em>.<\/p>\n<p>All laboratory personnel must read the contents of this manual and sign and date the Biosafety Manual Acknowledgement Form (found in Appendix A) which will be kept on file by the EPA Coordinator. By signing this signature page, the lab personnel agree to abide by the safety precautions and procedures discussed herein.<\/p>\n<h2>PURPOSE<\/h2>\n<p>The purpose of this manual is to specify controls and safe handling practices for microorganisms, biological toxins, recombinant or synthetic nucleic acid molecules, human blood or tissues, and animal cell cultures.<\/p>\n<h3>FIRE ALARMS AND EXTINGUISHERS<\/h3>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<p>It is the policy of Tusculum University that under reasonable\/appropriate circumstances suitably trained personnel may attempt to extinguish fires. All laboratories at Tusculum University are provided with carbon dioxide extinguishers. All fire extinguishers should be mounted on a wall or be standing vertically on the floor in an area free of clutter, normally near the entrance to the laboratory. Laboratory personnel should be familiar with the location and use of the fire extinguishers in their laboratory.<\/p>\n<p>For those departments wishing to train their employees on the proper use of fire extinguishers contact the CHO for setting up a training session. Any time a fire extinguisher is used, no matter for how brief a period, it should be inspected and recharged. For recharging, replacement, inspection, or information regarding the type of extinguisher best suited for your laboratory, contact the CHO. All fire extinguishers Tusculum University Campus are inspected monthly by campus safety to ensure they are in working order.<\/p>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h3>EYEWASHES<\/h3>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<p>All laboratories on Tusculum University Campus that contain hazardous substances must be equipped with an unobstructed safety shower and eyewash. These safety showers and eye wash facilities must meet the requirements of OSHA regulations (29 CFR l910.151(c), see Part XIII).<\/p>\n<p>Keep all passageways to the eyewash and shower clear of any obstacle (even a temporarily parked chemical cart). In the event of a chemical splash affecting the eye(s), immediate flush the eye(s) with copious amounts of water for 15 minutes. Medical evaluation should be sought for any\/all eye trauma.<\/p>\n<p>It is the responsibility of Chemical Hygiene Officers to inspect safety showers, and eyewash facilities annually and to flush each weekly (see Appendix E for Safety shower and eye wash inspection forms). A copy of the ANSI 2016 safety shower and eye wash inspection guideline can be found <a href=\"http:\/\/www.gesafety.com\/downloads\/ANSIGuide.pdf\" target=\"_blank\">here<\/a>.<\/p>\n<p>In the case of exposure, proceed to the nearest eyewash station. Hold eyelids open with thumb and forefinger and rinse for at least 15 minutes. Wash from outside edges towards the inside of the eye to prevent washing contaminants back into the eye. Rinse should be aimed at the inner corner of the eye (near the nose) and not directly at the eyeball. \u201cRoll\u201d eyes around and\u00a0up and down to ensure full rinsing. Have another member of the lab call for emergency response immediately. The area around the eye wash station must remain clear at all times.<\/p>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h2>INJURY TO AN INDIVIDUAL<\/h2>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<ol>\n<li>Immediately stop work and flush affected area with soap and water for 15 minutes.<\/li>\n<li>If the injury is a Medical Emergency call 911<\/li>\n<li>Secure all infectious materials.<\/li>\n<li>Notify Chemical Hygiene Officer. This is very important as Tusculum University maintains an ongoing log\/list of spills and injuries.<\/li>\n<li>Use the nearest First Aid Kit located in the laboratory.<\/li>\n<\/ol>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h2>DISINFECTION &amp; STERILIZATION<\/h2>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<p>Disinfectants reduce the number of pathogenic organisms. The following is a list of acceptable disinfectants and the microorganism they work against.<\/p>\n<ol>\n<li>Alcohols \u2013 Ethyl or isopropyl alcohol at 70-80% concentration is a good all purpose disinfectant. It is not effective against bacterial spores and non-enveloped viruses, such as adenovirus.<\/li>\n<li>Phenolic Compounds \u2013 effective against vegetative bacteria, fungi, and lipid-containing viruses. Less effective versus spores. Unpleasant odor (e.g. amphyl, Vesphene 2).<\/li>\n<li>Formaldehydes \u2013 At a concentration of 5-8% formalin, good disinfectant properties against vegetative bacteria, spores and viruses. Irritant sensitizer and animal carcinogen.<\/li>\n<li>Quaternary Ammonium Compounds (QUATs) \u2013 Acceptable as disinfectant to control vegetative bacteria and non-lipid containing viruses.<\/li>\n<li>Chlorine \u2013 Low concentrations (50-500 ppm) active against vegetative bacteria and most viruses. Higher concentration (2500 ppm) required for bacterial spores. Strong irritant. Corrosive to metal surfaces. Must be made up fresh (no residual action). Laundry bleach (5.25% chlorine), Alcide. 10 ml of laundry bleach per liter of water yields approximately 525 ppm.<\/li>\n<li>Iodofors \u2013 Recommended for general use (75-150 ppm). Effective against vegetative bacteria and viruses but poor activity against spores. Brown or yellow solution is still active. Wescodyne diluted 1 to 10 is popular disinfectant for washing hands.<\/li>\n<li>Glutaraldehydes \u2013 2% solutions exhibit good activity against vegetative bacteria, spores, and viruses. Toxic and capable of eye damage (e.g. Cides, Sporicidin, 3M Glutarex).<\/li>\n<\/ol>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h2>TRAINING<\/h2>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<p>All laboratory personnel must take institutionally provided training. Training must be documented (electronically or on paper). Personnel should not initiate research until training is completed. Copies of completed training certificated should be kept on file by the Chemical Hygiene Officer. Laboratory personnel are not allowed to work with agent(s) until they have been trained. The worker should demonstrate good microbiological skills and an understanding of this SOP prior to being permitted to work with agent(s).<\/p>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h2>BIOHAZARD WARNING SIGNS &amp; POSTING<\/h2>\n<div class=\"su-expand su-expand-collapsed su-expand-link-style-default\" data-height=\"0\"><div class=\"su-expand-content su-u-trim\" style=\"color:#333333;max-height:0px;overflow:hidden\">\n<p>Each laboratory must clearly display a sign that provides safety information to visitors and service personnel. All areas and laboratories which contain biohazardous agents must be posted with a biohazard sign. The sign must have information regarding the biosafety level, materials used, entry requirements, exit requirements, emergency contact name and phone number.<\/p>\n<p>An example of the biosafety sign to be placed on each door of a lab where microbiological agents will be is use can be found in the Appendix D.<\/p>\n<\/div><div class=\"su-expand-link su-expand-link-more\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show more<\/span><\/a><\/div><div class=\"su-expand-link su-expand-link-less\" style=\"text-align:left\"><a href=\"javascript:;\" style=\"color:#FF671B;border-color:#FF671B\"><span style=\"border-color:#FF671B\">Show less<\/span><\/a><\/div><\/div>\n<h2><a href=\"https:\/\/web.tusculum.edu\/safety\/wp-content\/uploads\/2020\/02\/Appendix-A.pdf\" target=\"_blank\">APPENDIX A<\/a><\/h2>\n<h2><a href=\"https:\/\/web.tusculum.edu\/safety\/wp-content\/uploads\/2020\/02\/Appendix-B.pdf\" target=\"_blank\">APPENDIX B<\/a><\/h2>\n<h2><a href=\"https:\/\/web.tusculum.edu\/safety\/wp-content\/uploads\/2020\/02\/Appendix-C.pdf\" target=\"_blank\">APPENDIX C<\/a><\/h2>\n<h2><a href=\"https:\/\/web.tusculum.edu\/safety\/wp-content\/uploads\/2020\/02\/Appendix-D.pdf\" target=\"_blank\">APPENDIX D<\/a><\/h2>\n<div class=\"su-button-center\"><a href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/biological-spills-in-the-laboratory\/\" class=\"su-button su-button-style-flat\" style=\"color:#FFFFFF;background-color:#FF671B;border-color:#cc5316;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:7px 20px;font-size:16px;line-height:24px;border-color:#ff9560;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-arrow-circle-right\" style=\"font-size:16px;color:#FFFFFF\"><\/i> BIOLOGICAL SPILLS IN THE LABORATORY<\/span><\/a><\/div>\n<p>&nbsp;<\/p>\n<div class=\"su-button-center\"><a href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/biological-waste-disposal\/\" class=\"su-button su-button-style-flat\" style=\"color:#FFFFFF;background-color:#FF671B;border-color:#cc5316;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:7px 20px;font-size:16px;line-height:24px;border-color:#ff9560;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-arrow-circle-right\" style=\"font-size:16px;color:#FFFFFF\"><\/i> BIOLOGICAL WASTE DISPOSAL<\/span><\/a><\/div>\n<p>&nbsp;<\/p>\n<div class=\"su-button-center\"><a href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/bsl-classification-of-agents\/\" class=\"su-button su-button-style-flat\" style=\"color:#FFFFFF;background-color:#FF671B;border-color:#cc5316;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:7px 20px;font-size:16px;line-height:24px;border-color:#ff9560;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-arrow-circle-right\" style=\"font-size:16px;color:#FFFFFF\"><\/i> BIOSAFETY LEVEL (BSL) CLASSIFICATION OF AGENTS<\/span><\/a><\/div>\n<p>&nbsp;<\/p>\n<div class=\"su-button-center\"><a href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/sop\/\" class=\"su-button su-button-style-flat\" style=\"color:#FFFFFF;background-color:#FF671B;border-color:#cc5316;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:7px 20px;font-size:16px;line-height:24px;border-color:#ff9560;border-radius:7px;-moz-border-radius:7px;-webkit-border-radius:7px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-arrow-circle-right\" style=\"font-size:16px;color:#FFFFFF\"><\/i> STANDARD OPERATING PROCEDURES<\/span><\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>TUSCULUM UNIVERSITY SAFETY INFORMATION AND EMERGENCY CONTACT INFORMATION Emergency Providers Laboratory Contact Information Reporting Incidents The following information will be needed when communicating with any emergency provider: Nature of the incident (description \u2013 including fire, substances involved, number of individuals involved, and their physical condition). Individuals involved (name, gender, age) Location of the incident (including [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":623,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"_links_to":"","_links_to_target":""},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v19.0 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>TUSCULUM COLLEGE :: BIOSAFETY MANUAL<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/web.tusculum.edu\/safety\/environmental-health-safety\/human-health\/biosafety\/biosafety-manual\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"TUSCULUM COLLEGE :: BIOSAFETY MANUAL\" \/>\n<meta property=\"og:description\" content=\"TUSCULUM UNIVERSITY SAFETY INFORMATION AND EMERGENCY CONTACT INFORMATION Emergency Providers Laboratory Contact Information Reporting Incidents The following information will be needed when communicating with any emergency provider: Nature of the incident (description \u2013 including fire, substances involved, number of individuals involved, and their physical condition). 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